Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 58, Issue 5, Pages 2763-2770Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf903274z
Keywords
Auxin; dispersive liquid-liquid microextraction; high-performance liquid chromatography; fluorescence detection
Funding
- National Nature Sciences Foundation of China [20675016, 20735002, 20877019]
- Key Program of Science and Technology Department of Fujian Province, China [200710020, 2007Y0060]
- Key Special Purpose Foundation of Physical Education Bureau of Fujian Province [HX2005-74]
- Plan of the University New Century Outstanding Talent of Fujian Province
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Auxin plays an important role in cell differentiation, apical dominance, and tropism in plants. A new method based on dispersive liquid-liquid microextraction (DLLME) combined with high-performance liquid chromatography-fluorescence detection (HPLC-FLD) has been established to detect auxin. A mixture of CHCl3 (extraction solvent) and acetone (disperser solvent) was injected quickly into a sample solution with desired salt concentration and pH value, and then a cloudy solution consisting of many dispersed fine droplets of CHCl3 was formed. After centrifugation, the sedimented phase was withdrawn and directly analyzed by HPLC-FLD. Under optimal conditions, four auxins were baseline separated within 3.5 min, with the minimal limit of detection of 0.02 ng mL(-1) and coefficient correlations in the range of 0.9980-0.9995. This simple method was successfully applied to real sample analysis. Experimental results showed that DLLME was a high-performance and powerful preconcentration method to extract and enrich related plant auxin.
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