Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 58, Issue 2, Pages 1305-1312Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf903336t
Keywords
Saffron; picrocrocin; cracetin esters; ISO quality standard
Funding
- European Community (EC) [030195-2]
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In this paper, 345 saffron samples were analyzed from different countries to study their picrocrocin content using different analytical techniques. The E-1 cm(1%) of 257 nm results from all samples are inflated in comparison by the high-performance liquid chromatography (HPLC) data, because of the interferences with the crocetin ester pool and especially with those with a lower trans/cis relation. A picrocrocin range update is proposed for International Organization for Standardization (ISO) 3632 normative because category III should be incremented up to 50 units, while category 11 should be incremented up to 60 units. More accurate data are achieved when the Delta E-pic, measurement is carried out. Consequently, improvements to the ISO method are suggested. Fourier transform (FT)near-infrared spectrometry analysis has also been carried out, showing excellent results from the calibration with HPLC data. This spectrophotometric technique could be used by saffron enterprises to obtain quick and more accurate data for picrocrocin determination.
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