Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 58, Issue 18, Pages 9979-9987Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf101942x
Keywords
Solanum lycopersicum; Solanum pimpinellifolium; plant cell culture; lycopene; phytoene; metabolic tracing; isotopic labeling; carotenoids; tomato; nutrition; metabolism
Funding
- National Institutes of Health National Cancer Institute (NIH/NCI) [CA 112649-01A1]
- University of Illinois College of ACES
- Division of Nutritional Sciences
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Isotopically labeled tomato carotenoids, phytoene, phytofluene, and lycopene, are needed for mammalian bioavailability and metabolism research but are currently commercially unavailable. The goals of this work were to establish and screen multiple in vitro tomato cell lines for carotenoid production, test the best producers with or without the bleaching herbicides, norflurazon and 2-(4-chlorophenyl-thio)-triethylamine (CPTA), and to use the greatest carotenoid accumulator for in vitro C-13-labeling. Different Solanum lycopersicum allelic variants for high lycopene and varying herbicide treatments were compared for carotenoid accumulation in callus and suspension culture, and cell suspension cultures of the hp-1 line were chosen for isotopic labeling. When grown with [U]-C-13-glucose and treated with CPTA, hp-1 suspensions yielded highly enriched C-13-lycopene with 45% of lycopene in the M+40 form and 88% in the M+35 to M+40 isotopomer range. To the authors' knowledge this is the first report of highly enriched C-13-carotenoid production from in vitro plant cell culture.
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