Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 57, Issue 15, Pages 6678-6684Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf901417k
Keywords
Rooibos; Aspalathus linearis; aspalathin; nothofagin; optimized geometric conformers; lipid peroxidation; metal chelation; herbal tea
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The antioxidant activity of rooibos flavonoids, including the dihydrochalcones aspalathin and nothofagin and their corresponding flavone glycosides, was evaluated using the ABTS radical cation, metal chelating, and Fe(II)-induced microsomal lipid peroxidation assays. Epigallocatechin gallate (EGCG) and Trolox were used as reference standards. Optimized geometric conformers of aspalathin and nothofagin, in addition to calculated physicochemical properties, were considered to explain interaction with the microsomal membrane structure and thus relative potency of the dihydrochalcones. The most potent radical scavengers were aspalathin (IC50 = 3.33 mu M) and EGCG (IC50 = 3.46 mu M), followed by quercetin (IC50 = 3.60 mu M) and nothofagin (IC50 = 4.04 mu M). The least effective radical scavengers were isovitexin (IC50 = 1224 mu M) and vitexin (IC50 > 2131 mu M). Quercetin (IC50 = 17.5 mu M) and EGCG (IC50 = 22.3 mu M) were the most effective inhibitors of lipid peroxidation. Aspalathin (IC50 = 50.2 mu M) and catechin (IC50 = 53.3 mu M) displayed similar potencies. Nothofagin (IC50 = 1388 mu M) was almost as ineffective as its flavone glycoside analogues.
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