4.7 Article

Obtention and Characterization of Phenolic Extracts from Different Cocoa Sources

Journal

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 56, Issue 20, Pages 9621-9627

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/jf8014415

Keywords

Antioxidant activity; cocoa sources; phenolic compound; DPPH; ORAC; UPLC-MS/MS

Funding

  1. Spanish Minister of Industry
  2. Catalan government (Interdepartmental Commission for Research and Technological Innovation)

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The aim of this study was to evaluate several cocoa sources to obtain a rich phenol extract for use as an ingredient in the food industry. Two types of phenolic extracts, complete and purified, from different cocoa sources (beans, nibs, liquor, and cocoa powder) were investigated. UPLC-MS/MS was used to identify and quantify the phenolic composition of the extracts, and the Folin-Ciocalteu and vanillin assays were used to determine the total phenolic and flavan-3-ol contents, respectively. The DPPH and ORAC assays were used to measure their antioxidant activity. The results of the analysis of the composition of the extracts revealed that the major fraction was procyanidins, followed by flavones and phenolic acids. From the obtained results, the nib could be considered the most interesting source for obtaining a rich phenolic cocoa extract because of its rich phenolic profile content and high antioxidant activity in comparison with the other cocoa sources.

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