Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 56, Issue 22, Pages 10651-10659Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jf801710a
Keywords
Antioxidant compounds; autohydrolysis; barley husks; resins; Sephadex; solvent extraction
Funding
- panish Ministry of Science of Technology [AGL2003-03596]
- FEDER funds of the European Union
- PGIDT [04PX138301PN]
- MEC [PTA-2003-02-00194]
Ask authors/readers for more resources
The liquid phase from nonisothermal autohydrolysis of barley husks was extracted with ethyl acetate and redissolved in ethanol to yield a crude extract (denoted BHEAE), which was subjected to further processing to enhance the antioxidant activity. A fractionation method, carried out for characterization purposes, consisted of the extraction of BHEAE with organic solvents of increasing polarity and further fractionation in Sephadex LH-20. Among the tested solvents, ethyl acetate allowed the highest yield, phenolic content, and antioxidant activity. Upon elution with methanol, products with high DPPH radical scavenging capacity (IC50 = 0.22 g/L) were obtained. The major compounds in the isolate were benzoic and cinnamic acids. Adsorption-desorption in commercial polymeric resins was carried out as an alternative strategy for BHEAE refining. This method is more suited for possible scale-up and provided a concentrate with a Trolox equivalent antioxidant capacity of 9 mM, which was obtained at a yield of 18 g/kg of barley husks.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available