Journal
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
Volume 791, Issue -, Pages 1-11Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mrgentox.2015.07.006
Keywords
Comet assay; DNA damage; Hemocytes; Malondialdehyde; Mitotic recombination; Wing-spot test
Funding
- FONDECYT-CONICYT [11110181]
- Direccion General de Investigacion y Postgrado
- Universidad Catolica de Temuco
- DGIP UCT CD
- MECESUP [UCT 0804]
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Copper oxide nanoparticles (CuONPs) are used as semiconductors, catalysts, gas sensors, and antimicrobial agents. We have used the comet and wing-spot assays in Drosophila melanogaster to assess the genotoxicity of CuONPs and ionic copper (CuSO4). Lipid peroxidation analysis was also performed (Thiobarbituric Acid Assay, TBARS). In larval hemocytes, both CuONPs and CuSO4 caused significant dose-dependent increases in DNA damage (comet assay). In the wing-spot assay, an increase in the frequency of mutant spots was observed in the wings of the adults; CuONPs were more effective than was CuSO4. Both agents induced TBARS; again, CuONPs were more active than was CuSO4. The results indicate that CuONPs are genotoxic in Drosophila, and these effects may be mediated by oxidative stress. Most of the effects appear to be related to the presence of copper ions. (C) 2015 Elsevier B.V. All rights reserved.
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