Journal
JORNAL BRASILEIRO DE PNEUMOLOGIA
Volume 37, Issue 5, Pages 664-668Publisher
SOC BRASILEIRA PNEUMOLOGIA TISIOLOGIA
DOI: 10.1590/S1806-37132011000500015
Keywords
Tuberculosis; Mycobacterium bovis; Polymerase chain reaction
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Funding
- Brazilian Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, National Council for Scientific and Technological Development)
- Institutos Nacionais de Ciencia e Tecnologia (INCT, National Institutes of Science and Technology)
- International Clinical Operational and Health Services Research and Training Award (ICOHRTA) Mandate [5 U2R TW006883-03]
- Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ, Rio de Janeiro Research Foundation)
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In 2005 and 2006, 8,121 clinical specimens submitted to the Mycobacteriology Laboratory of the Clementino Fraga Filho University Hospital/Thoracic Diseases Institute, in the city of Rio de Janeiro, Brazil, were inoculated on Lowenstein-Jensen medium containing glycerol and pyruvate. There were 79 mycobacteria isolates that presented growth only on pyruvate-containing medium, and those isolates were selected for the presumptive identification of Mycobacterium bovis. The selected isolates were screened with biochemical tests, PCR amplification (with the specific primers Rv0577 and Rv1510), and pyrazinamide susceptibility tests. All of the strains isolated showed specific phenotypical and genotypical patterns characteristic of M. tuberculosis, and no M. bovis strains were detected.
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