Journal
JAPANESE JOURNAL OF APPLIED PHYSICS
Volume 48, Issue 11, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1143/JJAP.48.117002
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- Exploratory Research Program for Young Scientists at Tohoku University
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In this paper we describe an organic solvent-free method for the in vitro fluorescence confocal imaging of living cells using nanocrystals of the hydrophobic fluorescent dyes 3,3'-dioctadecyloxacarbocyanine perchlorato (DiO) and perylene, which we obtained separately using the reprecipitation method. Confocal microscopy imaging revealed that cancer cells took up the dye with high avidity Under similar treatment conditions, the dyes in their nanocrystal forms or as dimethylsulfoxide solutions provided comparable in vitro fluorescence imaging of cells through confocal microscopy This nanocrystal formulation approach to in vitro bioimaging in cultures eliminates the need for external solubilizing agents (i.e, organic solvent, surfactant), which usually exhibit cytotoxicity Our developed technique also allowed the double-staining imaging of cancer cells using DiO and perylene nanocrystals (C) 2009 The Japan Society of Applied Physics DOI: 10.1143/JJAP.48.117002
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