4.7 Article

Long QT Syndrome-Associated Mutations in Intrauterine Fetal Death

Journal

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
Volume 309, Issue 14, Pages 1473-1482

Publisher

AMER MEDICAL ASSOC
DOI: 10.1001/jama.2013.3219

Keywords

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Funding

  1. Italian Ministry of Health [GR-2010-2305717]
  2. American Heart Association
  3. National Institutes of Health (NIH) [HD042569, HL087039, HL083374]
  4. Italian Ministero dell'Istruzione, dell'Universita e della Ricerca
  5. European Community
  6. T. Denny Sanford Endowed Collaborative Research Fund
  7. National Heart, Lung, and Blood Institute
  8. Institut Biochimique SA (IBSA) in Switzerland
  9. Ferring SA in Switzerland
  10. NIH
  11. Allergan Inc
  12. Gilead Sciences
  13. Fondazione IRCCS Policlinico S. Matteo, Pavia, Italy
  14. Mayo Clinic Windland Smith Rice Comprehensive Sudden Cardiac Death Program
  15. Sheikh Zayed Saif Mohammed Al Nahyan Fund in Pediatric Cardiology Research
  16. Dr Scholl Foundation
  17. Hannah M. Wernke Memorial Foundation

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Importance Intrauterine fetal death or stillbirth occurs in approximately 1 out of every 160 pregnancies and accounts for 50% of all perinatal deaths. Postmortem evaluation fails to elucidate an underlying cause in many cases. Long QT syndrome (LQTS) may contribute to this problem. Objective To determine the spectrum and prevalence of mutations in the 3 most common LQTS susceptible genes (KCNQ1, KCNH2, and SCN5A) for a cohort of unexplained cases. Design, Setting, and Patients In this case series, retrospective postmortem genetic testing was conducted on a convenience sample of 91 unexplained intrauterine fetal deaths (mean [SD] estimated gestational age at fetal death, 26.3 [8.7] weeks) that were collected from 2006-2012 by the Mayo Clinic, Rochester, Minnesota, or the Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. More than 1300 ostensibly healthy individuals served as controls. In addition, publicly available exome databases were assessed for the general population frequency of identified genetic variants. Main Outcomes and Measures Comprehensive mutational analyses of KCNQ1 (K(V)7.1, LQTS type 1), KCNH2 (HERG/K(V)11.1, LQTS type 2), and SCN5A (Na(V)1.5, LQTS type 3) were performed using denaturing high-performance liquid chromatography and direct DNA sequencing on genomic DNA extracted from decedent tissue. Functional analyses of novel mutations were performed using heterologous expression and patch-clamp recording. Results The 3 putative LQTS susceptibility missense mutations (KCNQ1, p.A283T; KCNQ1, p.R397W; and KCNH2[1b], p.R25W), with a heterozygous frequency of less than 0.05% in more than 10 000 publicly available exomes and absent in more than 1000 ethnically similar control patients, were discovered in 3 intrauterine fetal deaths (3.3% [95% CI, 0.68%-9.3%]). Both K(V)7.1-A283T (16-week male) and K(V)7.1-R397W (16-week female) mutations were associated with marked K(V)7.1 loss-of-function consistent with in utero LQTS type 1, whereas the HERG1b-R25W mutation (33.2-week male) exhibited a loss of function consistent with in utero LQTS type 2. In addition, 5 intrauterine fetal deaths hosted SCN5A rare nonsynonymous genetic variants (p.T220I, p.R1193Q, involving 2 cases, and p.P2006A, involving 2 cases) that conferred in vitro electrophysiological characteristics consistent with potentially pro-arrhythmic phenotypes. Conclusions and Relevance In this molecular genetic evaluation of 91 cases of intrauterine fetal death, missense mutations associated with LQTS susceptibility were discovered in 3 cases (3.3%) and overall, genetic variants leading to dysfunctional LQTS-associated ion channels in vitro were discovered in 8 cases (8.8%). These preliminary findings may provide insights into mechanisms of some cases of stillbirth. JAMA. 2013;309(14):1473-1482 www.jama.com

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