Journal
MOLECULES
Volume 20, Issue 10, Pages 19030-19040Publisher
MDPI
DOI: 10.3390/molecules201019030
Keywords
miRNA; stability; long freezing times; sequences AU or UA
Funding
- Regione Autonoma della Sardegna [L.R.7/2007]
- Fondazione Banco di Sardegna [998/2007.0729, 906/2012-1046]
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MicroRNAs (miRNAs) represent a family of small non-coding ribonucleic acids that post-transcriptionally inhibits the expression of their target messenger RNAs (mRNAs), thereby acting as general gene repressors. In this study we examined the relative quantity and stability of miRNA subjected to a long period of freezing; we compared the stability of eight miRNAs in the plasma of five human healthy controls before freezing and after six and 12 months of storage at -80 degrees C. In addition, we examined the plasma frozen for 14 years and the amount of miRNA still available. Using a Life Technologies protocol to amplify and quantify plasma miRNAs from EDTA (Ethylene Diamine Tetraacetic Acid)-treated blood, we analyzed the stability of eight miRNAs, (miR-125b-5p, miR-425-5p, miR-200b-5p, miR-200c-3p, miR-579-3p, miR-212-3p, miR-126-3p, and miR-21-5p). The miRNAs analyzed showed a high stability and long frozen half-life.
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