Journal
JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES
Volume 61, Issue 2, Pages 145-152Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/QAI.0b013e31825ddbbf
Keywords
IL-6; HIV-1 RNA; histocultures; macrophages; LPS; flagellin
Categories
Funding
- Center for AIDS Research at Case Western Reserve University [AI-36219]
- Tissue Procurement, Histology, and Immunohistochemistry Core Facility of the Case Comprehensive Cancer Center [CA43703]
- Fasenmyer Foundation
- National institute of Health [AI-76174]
- Bristol Myers Squibb
- GlaxoSmithKline
- Abbott
- Merck
- Gilead Sciences
- Pfizer
Ask authors/readers for more resources
Objective: Increased plasma IL-6 levels have been associated with HIV-1 disease progression risk, yet the drivers of IL-6 production in HIV-1 infection are not known. This study was designed to explore the relationship between HIV-1 replication and IL-6 induction. Design: Correlations between plasma levels of IL-6 and HIV-1 RNA were examined in 2 clinical studies. To more directly assess the induction of IL-6 by HIV-1, several cell and tissue types that support HIV-1 replication in vivo were infected with HIV-1, and expression of IL-6 was measured. Methods: Spearman rank correlations were used to examine the relationship between plasma levels of IL-6 and HIV-1 RNA. Macrophages and colonic and lymph node histocultures were infected with HIV-1 or stimulated with bacterial products, lipopolysaccharide (LPS) or flagellin, and IL-6 levels in supernatant were measured by enzyme-linked immunosorbent assay or multiplex bead assay. Results: In the clinical studies, there was weak or no correlation between plasma levels of IL-6 and HIV-1 RNA, but IL-6 levels were correlated with plasma levels of the LPS coreceptor CD14. Macrophages stimulated with LPS or flagellin showed robust production of IL-6, but there was no increase in IL-6 production after HIV-1 infection. IL-6 expression was not increased in lymph node histocultures obtained from HIV-1-infected subjects nor after productive HIV-1 infection of colonic or lymph node histocultures ex vivo. Conclusions: We find no evidence that HIV-1 replication is an important driver of IL-6 expression in vivo or in in vitro systems.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available