Journal
IUBMB LIFE
Volume 65, Issue 12, Pages 1026-1034Publisher
WILEY-BLACKWELL
DOI: 10.1002/iub.1231
Keywords
miRNA; miR-7; CUL5; cell cycle; hepatocellular carcinoma
Categories
Funding
- National Natural Science Foundation of China [91029714, 31071191, 31270818, 31101000]
- Natural Science Foundation of Tianjin [09JCZDJC175 00, 12JCZDJC25100]
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MicroRNAs (miRNAs) are small, non-coding RNAs that participate in the regulation of gene expression. In this study, we demonstrate that miR-7 was downregulated in hepatocellular carcinoma (HCC) tissues compared to adjacent non-tumor tissue. Over-expression of miR-7 in QGY-7703 and HepG2 cell lines inhibited colony formation and induced G1/S phase arrest, whereas knockdown of miR-7 produced the opposite phenotype. A tumor suppressor gene, CUL5, was identified as a direct target of miR-7, and CUL-5 is upregulated upon the binding of miR-7 to its 3UTR. Furthermore, suppression of CUL5 also suppressed cell colony formation and induced cell cycle arrest. Ectopic expression of CUL5 abrogated the effects of miR-7 inhibition on QGY-7703 and HepG2 cell lines. These results indicate that miR-7 suppresses colony formation and causes cell cycle arrest via upregulation of CUL5, and it may function as a tumor suppressor in HCC. (c) 2013 IUBMB Life, 65(12):1026-1034, 2013
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