Journal
IUBMB LIFE
Volume 64, Issue 7, Pages 628-635Publisher
WILEY-BLACKWELL
DOI: 10.1002/iub.1030
Keywords
miRNA; miR-181b; cAMP responsive element binding protein 1; cell growth; gastric carcinoma
Categories
Funding
- National Natural Science Foundation of China [30873017, 91029714, 31071191]
- Natural Science Foundation of Tianjin [08JCZDJC23300, 09JCZDJC17500]
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MicroRNAs are a class of small endogenous non-coding RNAs that function as post-transcriptional regulators. In our previous study, we found that miR-181b was significantly downregulated in human gastric adenocarcinoma tissue samples compared to the adjacent normal gastric tissues. In this study, we confirm the down-regulation of miR-181b in human gastric cancer cell lines versus the gastric epithelial cells. Overexpression of miR-181b suppressed the proliferation and colony formation rate of gastric cancer cells. miR-181b downregulated the expression of cAMP responsive element binding protein 1 (CREB1) by binding its 3' untranslated region. Overexpression of CREB1 counteracted the suppression of growth in gastric cancer cells caused by ectopic expression of miR-181b. These results indicate that miR-181b may function as a tumor suppressor in gastric adenocarcinoma cells through negative regulation of CREB1. (c) 2012 IUBMB IUBMB Life, 64(7): 628635, 2012
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