Journal
MOLECULAR SYSTEMS BIOLOGY
Volume 11, Issue 2, Pages -Publisher
WILEY
DOI: 10.15252/msb.20145808
Keywords
DNA sensing; IFIX; innate immunity; interactome; PYHIN
Categories
Funding
- NIH [DP1DA026192, R21AI102187, R21 HD073044]
- HFSPO award [RGY0079/2009-C]
- AHA Founder's predoctoral fellowship [14PRE18890044]
- NJCCR postdoctoral fellowship
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The human PYHIN proteins, AIM2, IFI16, IFIX, and MNDA, are critical regulators of immune response, transcription, apoptosis, and cell cycle. However, their protein interactions and underlying mechanisms remain largely uncharacterized. Here, we provide the interaction network for all PYHIN proteins and define a function in sensing of viral DNA for the previously uncharacterized IFIX protein. By designing a cell-based inducible system and integrating microscopy, immunoaffinity capture, quantitative mass spectrometry, and bioinformatics, we identify over 300 PYHIN interactions reflective of diverse functions, including DNA damage response, transcription regulation, intracellular signaling, and antiviral response. In view of the IFIX interaction with antiviral factors, including nuclear PML bodies, we further characterize IFIX and demonstrate its function in restricting herpesvirus replication. We discover that IFIX detects viral DNA in both the nucleus and cytoplasm, binding foreign DNA via its HIN domain in a sequence-non-specific manner. Furthermore, IFIX contributes to the induction of interferon response. Our results highlight the value of integrative proteomics in deducing protein function and establish IFIX as an antiviral DNA sensor important for mounting immune responses.
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