Journal
MOLECULAR PLANT
Volume 8, Issue 4, Pages 612-621Publisher
CELL PRESS
DOI: 10.1016/j.molp.2014.10.008
Keywords
Arabidopsis thaliana; bikinin; leaf-disk culture; transcriptome; tracheary element differentiation; signaling
Categories
Funding
- Ministry of Education, Science, Sports and Culture of Japan (NC-CARP project)
- Japan Society for the Promotion of Science [26891005, 23227001]
- Grants-in-Aid for Scientific Research [26891005, 23227001] Funding Source: KAKEN
Ask authors/readers for more resources
During vascular development, procambial and cambial cells give rise to xylem and phloem cells. Because the vascular tissue is deeply embedded, it has been difficult to analyze the processes of vascular development in detail. Here, we establish a novel in vitro experimental system in which vascular development is induced in Arabidopsis thaliana leaf-disk cultures using bikinin, an inhibitor of glycogen synthase kinase 3 proteins. Transcriptome analysis reveals that mesophyll cells in leaf disks synchronously turn into procambial cells and then differentiate into tracheary elements. Leaf-disk cultures from plants expressing the procambial cell markers TDRpro: GUS and TDRpro: YFP can be used for spatiotemporal visualization of procambial cell formation. Further analysis with the tdr mutant and TDIF (tracheary element differentiation inhibitory factor) indicates that the key signaling TDIF-TDR-GSK3s regulates xylem differentiation in leaf-disk cultures. This new culture system can be combined with analysis using the rich material resources for Arabidopsis including cell-marker lines and mutants, thus offering a powerful tool for analyzing xylem cell differentiation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available