Journal
MOLECULAR NEUROBIOLOGY
Volume 53, Issue 4, Pages 2480-2488Publisher
HUMANA PRESS INC
DOI: 10.1007/s12035-015-9225-z
Keywords
Tunneling nanotubes; Neural stem cells; Brain microvascular endothelial cells; MCAO model
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Funding
- National Natural Science Foundations of China [81230027, 81070959, 81471219]
- Outstanding Subject Leaders Project of Shanghai [14XD1403400]
- Technology Support Project of Shanghai [14140903300]
- Science Committee Animal Project of Shanghai [13140903400]
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Evidence indicates that neural stem cells (NSCs) can ameliorate cerebral ischemia in animal models. In this study, we investigated the mechanism underlying one of the neuroprotective effects of NSCs: tunneling nanotube (TNT) formation. We addressed whether the control of cell-to-cell communication processes between NSCs and brain microvascular endothelial cells (BMECs) and, particularly, the control of TNT formation could influence the rescue function of stem cells. In an attempt to mimic the cellular microenvironment in vitro, a co-culture system consisting of terminally differentiated BMECs from mice in a distressed state and NSCs was constructed. Additionally, engraftment experiments with infarcted mouse brains revealed that control of TNT formation influenced the effects of stem cell transplantation in vivo. In conclusion, our findings provide the first evidence that TNTs exist between NSCs and BMECs and that regulation of TNT formation alters cell function.
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