Association of hsa-miR-145 overexpression in human testicular cells with male infertility

MicroRNAs (miRs) have crucial functions in spermatogenesis and implications for male infertility. In the present study, Homo sapiens (hsa)-miR-145 was designed and cloned into the eukaryotic expression plasmid pGenesil-1. The recombinant plasmids were transfected into Hs 1.tes normal testicular cells and NTERA-2 testicular cancer cells. Quantitative polymerase chain reaction of hsa-miR-145 indicated that pGenesil-1-miR-145 effectively upregulated. the expression of hsa-miR-145 in vitro. hsa-miR-145 overexpression inhibited the mRNA and protein expression of sex-determining region Y Box 9 in Hs 1.tes cells. The proliferation rates of NTERA-2 cells transfected with pGenesil-1-miR-145 were significantly decreased. High expression levels of miR-145 promoted cell apoptosis in NTERA-2 cells. The results revealed that altered hsa-miR-145 expression in testicular cells affects the regulation of target genes associated with male infertility.