Sphingobacterium haloxyli sp nov., an endophytic bacterium isolated from Haloxylon ammodendron stems in Kumtag desert

A Gram-stain-negative, non-motile, aerobic, non-spore-forming, rod-shaped, bacterial strain, designated 5JN-11(T), was isolated from Haloxylonammodendron stems in Kumtag desert, Xinjiang province, China. Strain 5JN-11(T) grew at salinities of 0-6 % (w/v; optimum 0-2 %), a pH of 7.0-9.0 (pH 7.0-8.0) and temperatures of 20-42 degrees C (28-30 degrees C). Based on 16S rRNA gene sequences, the strain was designated a member of the genus Sphingobacterium and the phylogenetic analysis showed that strain 5JN-11(T) shared the highest similarity to Sphingobacterium gobiense H7(T), followed by Sphingobacterium chuzhouense DH-5(T) and Sphingobacterium arenae H-12(T). The unfinished draft genome of strain 5JN-11(T) was 4.69 Mb. The G+C content of strain 5JN-11(T) was 42.8 mol%. The average nucleotide identity to S. gobiense H7(T) was 90.5 %. The respiratory quinone was MK-7, and the major polar lipids were phosphatidylethanolamine and phosphoglycolipid. The predominant cellular fatty acids were summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), iso-C-15:0 and iso-C-17:0 3-OH. On the basis of phenotypic, genotypic and phylogenetic evidence, strain 5JN-11(T) represents a novel species in the genus Sphingobacterium, for which the name Sphingobacterium haloxyli sp. nov. is proposed. The type strain is 5JN-11(T) (=ACCC 60072(T)=KCIC 62457(T)).