Chitinophaga parva sp nov., a new member of the family Chitinophagaceae, isolated from soil in a chemical factory

A Gram-stain-negative bacterium, designated LY-1(T), was isolated from the soil sample collected from a chemical factory in Fuyang city, Anhui province, China. Cells of strain LY-1(T) were strictly aerobic, non-motile and rod-shaped. Strain LY-1(T) grew optimally at pH 7.0 and at 30-35 degrees C. The taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LY-1(T) was a member of the genus Chitinophaga and showed the highest sequence similarity to Chitinophaga costaii A37T2(T) (97.5 %) and lower (< 97.0 %) sequence similarity to other known Chitinophaga species. Chemotaxonomic analysis revealed that strain LY-1(T) possessed menaquinone-7 as the major isoprenoid quinone; and iso-C-15:0 (46.4 %), C-16:1 omega 5c (27.8 %) and iso-C-17:0 3-OH (9.0 %) were the predominant fatty acids. The polar lipids of strain LY-1(T) consisted of phosphatidylethanolamine, three unidentified phosphoaminolipids, one unidentified phospholipid, four unidentified lipids, two unidentified aminolipids and two unidentified glycolipids. The genomic DNA G+C content of strain LY-1(T) was 52.4 mol% based on total genome calculations. The average nucleotide identity and the digital DNA-DNA hybridization value of the draft genomes between strain LY-1(T) and strain A37T2(T) were 76.8 and 19.8 %, respectively. Based on the phylogenetic and phenotypic characteristics, chemotaxonomic data, and DNA-DNA hybridization, strain LY-1(T) is considered a novel species of the genus Chitinophaga, for which the name Chitinophaga parva sp. nov. (type strain LY-1(T) = CCTCC AB 2018018(T)=KCTC 62444(T)) is proposed.