Journal
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Volume 64, Issue -, Pages 1882-1889Publisher
SOC GENERAL MICROBIOLOGY
DOI: 10.1099/ijs.0.052720-0
Keywords
-
Categories
Funding
- USDA NIFA Special Research Grant [2010-34459-20756]
- USDA NIFA National Integrated Food Safety Initiative Grants [2008-51110-04333, 2008-51110-04688]
- NIFA [583429, 2008-51110-04688, 583539, 2008-51110-04333] Funding Source: Federal RePORTER
Ask authors/readers for more resources
Sampling of agricultural and natural environments in two US states (Colorado and Florida) yielded 18 Listeria-like isolates that could not be assigned to previously described species using traditional methods. Using whole-genome sequencing and traditional phenotypic methods, we identified five novel species, each with a genome-wide average BLAST nucleotide identity (ANIb) of less than 85% to currently described species. Phylogenetic analysis based on 16S rRNA gene sequences and amino acid sequences of 31 conserved loci showed the existence of four well-supported clades within the genus Listeria; (i) a clade representing Listeria monocytogenes, L. marthii, L. innocua, L. welshimeri, L. seeligeri and L. ivanovii, which we refer to as Listeria sensu stricto, (ii) a clade consisting of Listeria fleischmannii and two newly described species, Listeria aquatica sp. nov. (type strain FSL S10-1188(T)=DSM 26686(T)=LMG 28120(T)=BEI NR-42633(T)) and Listeria floridensis sp. nov. (type strain FSL S10-1187(T)=DSM 26687(T)=LMG 28121(T)=BEI NR-42632(T)), (iii) a clade consisting of Listeria rocourtiae, L. weihenstephanensis and three novel species, Listeria cornellensis sp. nov. (type strain TTU A1-0210(T)=FSL F6-0969(T)=DSM 26689(T)=LMG 28123(T)=BEI NR-42630(T)), Listeria grandensis sp. nov. (type strain TTU A1-0212(T)=FSL F6-0971(T)=DSM 26688(T)=LMG 28122(T)=BEI NR-42631(T)) and Listeria riparia sp. nov. (type strain FSL S10-1204(T)=DSM 26685(T)=LMG 28119(T)=BEI NR-42634(T)) and (iv) a clade containing Listeria grayi. Genomic and phenotypic data suggest that the novel species are non-pathogenic.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available