Phenylobacterium kunshanense sp nov., isolated from the sludge of a pesticide manufacturing factory

A novel aerobic, Gram-stain-negative, motile bacterium, designated strain BUT-10(T), was isolated from the sludge of a pesticide manufacturing factory in Kunshan, China. Cells were rod-shaped (0.4-0.45x0.9-1.4 mu m) and colonies were white, circular with entire edges and had a smooth surface. The strain grew at 25-37 degrees C, at pH 6.0-8.0 and with 0-0.5% NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain BUT-10(T) was a member of the genus Phenylobacterium, and showed highest sequence similarities to Phenylobacterium muchangponense A8(T) (97.49%), Phenylobacterium immobile DSM 1986(T) (97.14%) and Phenylobacterium lituiforme Fail3(T) (96.34%). Major fatty acids (>5%) were summed feature 8 (comprising C-18:1 omega 7c and/or C-18:1 omega 6c), C-16:0 and gummed feature 3 (comprising C-16:1 omega 7c and/or C-16:1 omega 6c). The major isoprenoid quinone was ubiquinone-10. The DNA G+C content was 71.85 mol%. Strain BUT-10(T) showed low DNA DNA relatedness with P. muchangponense A8(T) (15.7 +/- 2.9%) and P. immobile DSM 1986(T) (12.8 +/- 1.1 %). On the basis of the phenotypic, phylogenetic and genotypic data, strain BUT-10(T) is considered to represent a novel species of the genus Phenylobacterium, for which the name Phenylobacterium kunshanense sp. nov. is proposed. The type strain is BUT-10(T) (=CCTCC AB 2013085(T)=KCTC 42014(T)).