Flavobacterium maotaiense sp nov., isolated from freshwater

Two novel strains, T9(T) and T10, were isolated from water samples collected from Chishui River flowing through Maotai town, Guizhou, south-west China. The isolates were yellow-pigmented, Gram-reaction-negative, rod-shaped, non-motile and aerobic. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates belonged to the genus Flavobacterium, and showed highest similarities to Flavobacterium hibemum DSM 12611(T) (97.0 %), followed by Flavobacterium granuli Kw05(T) (96.7%) and Flavobacterium pectinovorum DSM 6368(T) (96.7 %). The novel strains were able to grow at 20-37 degrees C (optimum 25 degrees C), pH 7.0-10.0 (optimum pH 7.0-8.0) and with 0-0.5% (w/v) NaCl (optimum 0.5%). The predominant fatty acids were iso-C-15:0, C-16:1 omega 7c, anteiso-C-15:0, C-15:0, iso-C-15:0 3-OH and iso-C-15:1 omega 10c, and menaquinone-6 (MK-6) was the main respiratory quinone. The major polar lipids were phosphatidylethanolamine, one unknown glycolipid, two unknown aminolipids and two unidentified lipids. The DNA G+C contents of strains T9(T) and T10 were 37.7 and 36.4 mol%, respectively. According to the phenotypic and genetic data, strains T9(T) and T10 represent a novel species in the genus Flavobacterium, for which the name Flavobacterium maotaiense sp. nov. is proposed. The type strain is T9(T) (=CGMCC 1.12712(T)=JCM 19927(T)).