Cohnella nanjingensis sp nov., an extracellular polysaccharide-producing bacterium isolated from soil

A Gram-stain-positive, rod-shaped, endospore-forming bacterium, strain D45(T), was isolated from soil in Nanjing, China. The organism grew optimally at 30 C, pH 7.0 and with 0% NaCI (w/v). The 16S rRNA gene sequence of the isolate showed similarities lower than 97% with respect to species of the genus Cohnella. The predominant respiratory quinone was MK-7, with MK-6 present as a minor component; anteiso-C15:0 and iso-C16,0 were the major fatty acids. The polar lipids of strain D45(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, four phospholipids, two glycolipids, one aminolipid and two lipids. The DNA G+C content was 59.5 mol%. DNA-DNA hybridization of the isolate with two reference strains showed relatedness values of 33.4 % with Cohnella ginsengisoli DSM 18997(T) and 25.8% with Cohnella thermotolerans DSM 17683(T). The phylogenetic, chemotaxonomic and phenotypic data supported the classification of strain D45(T) as a representative of a novel'species of the genus Cohnella, for which the name Cohnella nanjingensis sp. nov. is proposed. The type strain is D45(T) (=--CCTCC AB 2014067T=DSM 28246T).