Loktanella sediminum sp nov., isolated from marine surface sediment

A Gram-staining-negative, strictly aerobic and short rod-shaped bacterium, designated strain S3B03(T), was isolated from the sediment of the northern Okinawa Trough. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S3B03(T) belonged to the genus Loktanella (family Rhodobacteraceae) and showed the highest sequence similarity with Loktanella litorea KCTC 23883(T) (96.16%) and 92.99-95.90% 16S rRNA gene sequence similarity to other members of the genus Loktanella. Optimal growth occurred in the presence of 2-5% (w/v) NaCl at pH 7.0-8.0 and 28-32 degrees C. Ubiquinone-10 (Q-10) was the predominant respiratory quinone. The major fatty acids (>10% of the total fatty acids) were C-18:1 omega 7c and/or C-18:1 omega 6c. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and two unidentified polar lipids. The DNA G+C content of strain S3B03(T) was 57.6 mol%. On basis of polyphasic analysis, strain S3B03(T) is considered to represent a novel species of the genus Loktanella, for which the name Loktanella sediminum sp. nov. is proposed. The type strain is S3B03(T) (=JCM 30120(T)=DSM 28715(T)=MCCC 1K00257(T)).