Tenacibaculum xiamenense sp nov., an algicidal bacterium isolated from coastal seawater

A Gram-stain-negative, elongated rod-shaped, yellow-pigmented, aerobic bacterial strain, designated WJ-1(T), was isolated from coastal seawater in Xiamen, Fujian province, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain WJ-1(T) fell within the genus Tenacibaculum and was most closely associated with Tenacibaculum aestuarii SMK-4(T) (96.7% 16S rRNA gene sequence similarity); lower similarities were shown to other members of the genus Tenacibaculum (<96.2%). The strain formed a distinct lineage with Tenacibaculum litopenaei B-I-T (96.0 %), Tenacibaculum geojense YCS-6(T) (94.5 %) and Tenacibaculum jejuense CNURIC 013(T) (95.4%). Growth was observed at temperatures from 16 to 38 degrees C, at salinities from 2 to 4% and at pH 6-9. The major fatty acids were summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c), iso-C-17:0 3-OH, iso-C-15:0 and iso-C-15:0 3-OH. The DNA G+C content of strain WJ-1(T) was 33.2 mol% and the major respiratory quinone was menaquinone-6 (MK-6). Differential phenotypic properties and phylogenetic distinctiveness in this study distinguished strain WJ-1(T) from all other members of the genus Tenacibaculum. According to the morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, strain WJ-1(T) represents a novel species of the genus Tenacibaculum, for which the name Tenacibaculum xiamenense sp. nov. is proposed. The type strain is WJ-1(T) (=CGMCC 1.12378(T)=LMG 27422(T)).