Coprobacter fastidiosus gen. nov., sp nov., a novel member of the family Porphyromonadaceae isolated from infant faeces

A novel obligately anaerobic, non-spore-forming, rod-shaped, non-motile Gram-reaction-negative bacterium was isolated from infant faeces. The strain, designated NSB1(T), was able to grow on rich media at 30-37 degrees C, in the presence of up to 2% (w/v) Oxgall and 2% (w/v) NaCl. Cells of strain NSB1(T) produced catalase, but not urease and indole. Aesculin was not hydrolysed. The strain was able to utilize D-glucose, lactose, maltose, mannose and raffinose as electron donors. When grown on D-glucose, the main metabolic end products were propionic and acetic acids, with a minor product being succinic acid. The major cellular fatty acids, iso-C-15:0 and anteiso-C-15:0, were present at a 1 : 1 molar ratio. The major menaquinone was MK-11. The DNA G+C content was found to be 38.5 mol%. According to 16S rRNA gene sequence analysis strain NSB1(T) is a member of the family Porphyromonadaceae, phylum Bacteroidetes. The closest relatives of the strain were Bamesiella viscericola (88.2 % identity) and Barnesiella intestinihominis (87.4% identity). On the basis of phenotypic and genotypic properties of strain NSB1(T) we conclude that this strain represent a novel species in a new genus within the family of Porphyromonadaceae for which the name Coprobacter fastidiosus gen. nov., sp. nov. is proposed. The type strain of the species is NSB1(T) (=DSM 26242(T),=VKM B-2743(T)).