Mucilaginibacter gynuensis sp nov., isolated from rotten wood

A Gram-staining-negative, rod-shaped, aerobic bacterial strain designated YC7003(T), was isolated from a piece of rotten wood collected at Jinju, Korea. The taxonomic position of the strain was investigated using a polyphasic approach. The strain was catalase- and oxidase-positive, grew at 4-35 degrees C (optimum, 30 degrees C) and at pH 5.0-10.0 (optimum, pH 6.5-7.0). The major cellular fatty acids were C-16:1 omega 7c and/or iso-C-15:0 2-OH (summed feature 3), iso-C-15:0 and C-16 : 1 omega 5c and the major respiratory quinone was MK-7. The total genomic DNA G+C content was 49.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YC7003(T) belonged to the genus Mucilaginibacter in the family Sphingobacteriaceae with 94.4-97.2% sequence similarities with type strains of species of the genus Mucilaginibacter. The most closely related species was Mucilaginibacter mallensis MP1X4(T) (97.2%). The DNA-DNA relatedness value between strain YC7003(T) and M. mallensis MP1X4(T) was 21.7 +/- 3.3%. Based on these data, strain YC7003(T) represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter gynuensis sp. nov. is proposed. The type strain is YC7003(T) (=KACC 15532(T)=JCM 17705(T)).