Hirschia litorea sp nov., isolated from seashore sediment, and emended description of the genus Hirschia

A Gram-negative, non-spore-forming, non-flagellated, coccoid-, oval- or rod-shaped strain, designated M-M23(T), was isolated from seashore sediment at Geoje island, South Korea. Strain M-M23(T) grew optimally at 25 degrees C, at pH 7.0-8.0 and in the presence of 2% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain M-M23(T) clustered with the type strains of the two species of the genus Hirschia, with which it exhibited 97.6-98.1 % 16S rRNA gene sequence similarity. Sequence similarity with the type strains of other recognized species was <90.8%. Strain M-M23(T) contained Q-10 as the predominant ubiquinone and C-18:1 omega 7c and C-16:0 as the major fatty acids. The major polar lipids of strain M-M23(T) were phosphatidylglycerol and two unidentified lipids. The DNA G+C content of strain M-M23(T) was 45.4 mol%. DNA-DNA relatedness between the isolate and Hirschia baltica DSM 5838(T) and Hirschia maritima JCM 14974(T) was 22 +/- 7.2 and 14 +/- 5.6 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain M-M23(T) is separate from the other described members of the genus Hirschia. On the basis of the data presented, strain M-M23(T) is considered to represent a novel species of the genus Hirschia, for which the name Hirschia litorea sp. nov. is proposed. The type strain is M-M23(T) (=KCTC 32081(T) =CCUG 62793(T)). An emended description of the genus Hirschia is also provided.