Salinarimonas ramus sp nov and Tessaracoccus oleiagri sp nov., isolated from a crude oil-contaminated saline soil

Four bacterial strains, SL014B-41A4(T), SL014B-20A1(T), SL014B-76A1 and SL014B-79A, isolated from a crude oil-contaminated saline soil of Shengli Oilfield, China, were investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SL014B-41A4(T) belonged to the genus Salinarimonas in the order Rhizobiales, with the highest sequence similarity with Salinarimonas rosea YIM YD3(T) (98.3%). The DNA DNA relatedness of strain SL014B-41A4(T) to S. rosea YIM YD3(T) was 27.03 +/- 3.0%. Strain SL014B-41A4(T) was Gram-negative staining, facultatively anaerobic and produced deep red pigment in artificial seawater medium. Cells of strain SL014B-41A4(T) were rod-shaped (0.6-4.0x1.25-25 mu m), motile with a single polar flagellum and often formed branches. The strain contained Q-10 as the predominant respiratory ubiquinone and C(18:1 omega)17C (57.5%), C-16:0 (16.4 %) and 10-methyl C-19:0 (9.1 %) as the major fatty acids. Strains SL014B-20A1(T), SL014B-76A1 and SL014B-79A were actinobacteria and belonged to the genus Tessaracoccus in the family Propionibacteriaceae of the order Actinomycetales with the highest 16S rRNA gene sequence similarities with Tessaracoccus flavescens SST-39(T) (96.4%), Tessaracoccus lubricantis KISS-17Se(T) (96.2%) and Tessaracoccus bendigoensis Ben 106(T) (94.7%). Strains SL014B-20A1(T), SL014B-76A1 and SL014B-79A were Gram-positive staining, facultatively anaerobic, non-endospore-forming, non-motile, acid-fast and oval to rod-shaped (0.48x0.5-1.0 mu m). These three novel strains had LL-diaminopimelic acid (DAP) as the diagnostic diamino acid in the cell-wall peptidoglycan, MK-9(H-4) as the only menaquinone and anteiso-C-15:0 (67.11-76.14%) as the major cellular fatty acid. The G+C contents of the genomic DNA of strain SL014B-41A4(T) and strains SL014B-20A1(T), SL014B-76A1 and SL014B-79A were 67.68 mol% and 65.65-67.17 mol%, respectively. Based on phenotypic and genotypic characteristics, strain SL014B-41A4(T) represents a novel species of the genus Salinarimonas, for which the name Salinarimonas ramus is proposed, with strain SL014B-41A4(T) (=DSM 22962(T)=CGMCC 1.9161(T)) as the type strain. Strains SL014B-20A1(T), SL014B-76A1 and SL014B-79A represent a novel species of the genus Tessaracoccus, for which the name Tessaracoccus oleiagri is proposed, with strain SL014B-20A1(T) (=DSM 22955(T)=CGMCC 1.9159(T)) as the type strain.