Amycolatopsis samaneae sp. nov., isolated from roots of Samanea saman (Jacq.) Merr.

A novel actinomycete, designated strain RM287(T), was isolated from surface-sterilized roots of Samanea saman (Jacq.) Merr., collected from Bangkok, Thailand. The status of the novel strain was determined using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the organism formed a distinct phyletic line within the radiation of the genus Amycolatopsis. The 16S rRNA gene sequence similarity indicated that strain RM287(T) was most closely related to Amycolatopsis mediterranei IMSNU 20056(T) (97.4%), A. rifamycinica DSM 46095(T) (97.2%), A. kentuckyensis NRRL B-24129(T) (97.2%), A. pretoriensis DSM 44654(T) (97%) and A. australiensis DSM 44671(T) (97%). The novel organism was found to have chemical properties typical of members of the genus Amycolatopsis such as meso-diaminopimelic acid as the dignostic diamino acid in the cell-wall peptidoglycan and arabinose and galactose as the diagnostic sugars. The major menaquinone was MK-9(H(4)). The major fatty acids were iso-C(16:0) iso-C(15:0), iso 2-OH-C(16:0) and iso-C(17:0). The DNA G+C content was 71.7 mol%. Phenotypic data clearly distinguished the novel isolate from its closest relatives. The combined genotypic and phenotypic data indicated that strain RM287(T) represented a novel species of the genus Amycolatopsis. The proposed name for this organism is Amycolatopsis samaneae sp. nov., with the type strain RM287(T) (=TISTR 1919(T)=BCC 35842(T)=NBRC 106095(T)).