Roseovarius marinus sp. nov., isolated from seawater

A Gram-stain-negative, non-motile, ovoid- to rod-shaped bacterium, designated HDW-9(T), belonging to the class Alphaproteobacteria, was isolated from seawater of the Yellow Sea, Korea. Strain HDW-9(T) grew optimally at pH 7.0-8.0, at 30 degrees C and with 2-3% (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 165 rRNA gene sequences showed that strain HDW-9(T) clustered with Roseovarius crassostreae CV919-312(T), with which it exhibited 95.5% 16S rRNA gene sequence similarity. Strain HDW-9(T) exhibited 92.5-94.7% 16S rRNA gene sequence similarity with the other type strains of species of the genus Roseovarius. Strain HDW-9(T) contained Q-10 as the predominant ubiquinone and C-18:1 omega 7c as the major fatty acid. The DNA G+C content was 58.3 mol%. Differential phenotypic properties distinguished strain HDW-9(T) from the other members of the genus Roseovarius. Strain HDW-9(T) is considered to represent a novel species of the genus Roseovarius, for which the name Roseovarius marinus sp. nov. is proposed. The type strain is HDW-9(T) (=KCTC 22805(T) =CCUG 58403(T)).