Amycolicicoccus subflavus gen. nov., sp nov., an actinomycete isolated from a saline soil contaminated by crude oil

Two novel actinomycetes, designated DQS3-9A1(T) and DQS3-9A2, were isolated from a saline soil contaminated with crude oil in the Shengli Oilfield in China. On the basis of 16S rRNA gene sequence analysis, the two strains were most closely related to Mycobacterium species (92.7-94.9% similarities), and formed a distinct lineage in the suborder Corynebacterineae. In addition, the major sugars in the cell wall, arabinose and galactose, supported the affiliation of strain DQS3-9A1(T) with members of the family Mycobacteriaceae. However, strain DQS3-9A1(T) did not contain mycolic acids and MK-8 (85.5%) was the major menaquinone for both isolates. The major cellular fatty acids for strain DQS3-9A1(T) were C(16:0) (20.5%), 10-methyl C(17:0) (19.3%), 10-methyl C(18:0) (16.1%), summed feature 3 (11.4%) C(15:0) (11.3%), C(17:0) (5.0%) and C(17:1)omega 8c (5.0%). The polar lipids of strain DQS3-9A1(T) consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and an unknown glucosamine-containing phospholipid. These chemotaxonomic data indicated that strain DQS3-9A1(T) differs from the present members of the suborder Corynebacterineae. Therefore, the creation of Amycolicicoccus subflavus gen. nov., sp. nov. is proposed, with DQS3-9A1(T) (= DSM 45089(T) = CGMCC 4.3532(T)) as the type strain.