Oceanobacillus kapialis sp nov., from fermented shrimp paste in Thailand

A Gram-positive, rod-shaped, strictly aerobic, spore-forming, moderately halophilic bacterium, designated strain SSK2-2(T), was isolated from fermented shrimp paste (ka-pi) produced in Thailand. It contained MK-7 as the predominant menaquinone and meso-diaminopimelic acid in the cell-wall peptidoglycan. The isolate grew at 8-43 degrees C, pH 6-9 and in 0.5-24% (w/v) NaCl (optimum, 6-14% Nacl). The major cellular fatty acids were anteiso-C-15:0 and anteiso-C-17:0. Phosphatidylglycerol and diphosphatidylglycerol were the major polar lipid components. The DNA G + C content was 39.7 mol%. Comparative 16S rRNA gene sequence analyses showed that strain SSK2-2(T) was most closely related to Oceanobacillus picturae KCTC 3821(T) with 98.7% sequence similarity. Based on phenotypic and molecular features combined with DNA-DNA hybridization results (<= 24.9% with O. picturae KCTC 3821(T)), this strain represents a novel species of the genus Oceanobacillus for which the name Oceanobacillus kapialis sp. nov. is proposed; the type strain is SSK2-2(T) (=KCTC 13177(T)=PCU 300(T) =TISTR 1858(T)).