Journal
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Volume 59, Issue -, Pages 1579-1582Publisher
MICROBIOLOGY SOC
DOI: 10.1099/ijs.0.001826-0
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Funding
- National Natural Science Foundation of China [40376041]
- National Infrastructure of Natural Resources for Science and Technology Program of China [2005DKA21209]
- COMRA program [DYXCM115-02-2-05]
- National Basic Research Program of China [2004CB719601, 2006CB708200]
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A taxonomic study was carried out on a strain, designated W3-3A(T), which was isolated from a pyrene-degrading consortium, enriched from sediment of the Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain W3-3A(T) belonged to the genus Bowmanella, with the highest sequence similarity (99.0%) with Bowmanella denitrificans BD1(T), whereas sequence similarities with other species were less than 93%. The nucleotide sequence similarity of both gyrB and rpoD genes of strain W3-3A(T) and B. denitrificans BD1(T) was 81.1%. However, the protein sequence similarities of the gyrB and rpoD genes of strain W3-3A(T) and B. denitrificans BD1(T) were 96.1% and 91.0%, respectively. Phylogenetic trees based on these housekeeping genes showed that strain W3-3A(T) and B. denitrificans BD1(T) formed a distinct lineage in the Gammaproteobacteria. The DNA-DNA hybridization value between strain W3-3A(T) and B. denitrificans BD1(T) was 43%. Strain W3-3A(T) could also be differentiated from B. denitrificans BD1(T) based on the repetitive extragenic palindromic DNA-PCR fingerprint. The G+C content of the chromosomal DNA of strain W3-3A(T) was 49 mol%. The combined genotypic and phenotypic data showed that strain W3-3A(T) represents a novel species of the genus Bowmanella, for which the name Bowmanella pacifica sp. nov. is proposed, with the type strain W3-3A(T) (=CGMCC 1.7086(T)=LMG 24568(T)=MCCC 1A01018(T)).
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