Marinimicrobium locisalis sp nov., isolated from a marine solar saltern, and emended description of the genus Marinimicrobium

A Gram-negative, motile and rod-shaped bacterial strain, designated ISL-43(T), was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain ISL-43(T) grew optimally at pH 7.0-8.0 and 30 degrees C and in the presence of approximately 2% NaCl. It contained Q-8 as the predominant ubiquinone and C(16:0), C(19:0)omega 8C, C(16:1)omega 7c and/or iso-C(15:0) 2-OH, and C(18:1)omega 7c as the major fatty acids. The DNA G + C content was 58.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ISL-43(T) fell within the genus Marinimicrobium, clustering with Marinimicrobium agarilyticum M18(T) with a bootstrap value of 100%. Strain ISL-43(T) exhibited 16S rRNA gene sequence similarity values of 98.1 and 95.5% to M. agarilyticum M18(T) and Marinimicrobium koreense M9(T), respectively. Strain ISL-43(T) exhibited DNA-DNA relatedness values of 17 and 10% to M. agarilyticum KCTC 12357(T) and M. koreense KCTC 12356(T), respectively. On the basis of phenotypic, phylogenetic and genetic data, strain ISL-43(T) represents a novel species within the genus Marinimicrobium, for which the name Marinimicrobium locisalis sp. nov. is proposed. The type strain is ISL-43(T) (=KCTC 22484(T) =CCUG 56757(T)).