Nitratireductor kimnyeongensis sp nov., isolated from seaweed

An aerobic, Gram-negative, catalase-positive, oxidase-positive bacterium was isolated from a dried seaweed sample collected from Kimnyeong Beach in Jeju, Republic of Korea. The cells of the organism, designated strain KY 101(T), were rods (0.4-0.5x1.2-2.7 mu m) and motile by means of flagella. The colonies of the cells were 0.5-1 mm in diameter, smooth, circular, convex and light yellow in colour. The isolate showed growth at 10-40 degrees C, pH 6.1-12.1 and in the presence of 7% NaCl. The major fatty acid was summed feature C-18:1 omega 7c/C-18:1 omega 9c/C-18:1 omega 12t and the DNA G + C content was 60.4 mol%. 16S rRNA gene sequence studies showed that the organism was phylogenetically related to the family Phyllobacteriaceae, with Nitratireductor aquibiodomus (99.1% sequence similarity) as the closest neighbour. The level of DNA-DNA relatedness between strain KY 101(T) and N. aquibiodomus DSM 15645(T) was 18.4-23.2% (duplicate measurements). The combination of phenotypic differences and DNA-DNA hybridization data supports the suggestion that the isolate represents a novel species of the genus Nitratireductor, for which the name Nitratireductor kimnyeongensis sp. nov. is proposed. The type strain is strain KY 101(T) (=KACC 11904(T)=JCM 14851(T)).