Caldicellulosiruptor kronotskyensis sp nov and Caldicellulosiruptor hydrothermalis sp nov., two extremely thermophilic, cellulolytic, anaerobic bacteria from Kamchatka thermal springs

Five novel strains (2002(T), 2902, 2006, 108(T) and 117) of cellulose-degrading, anaerobic, thermophilic bacteria were isolated from terrestrial hot springs of Kamchatka (Far East, Russia). Strains 2002 T and 108(T) were non-spore-forming bacteria with a Gram-positive type cell wall and peritrichous flagella. Optimum growth of strains 2002(T)and 108(T) occurred at pH 7.0 and at temperatures of 70 and 65 degrees C, respectively. The G + C contents of the DNA of strains 2002(T) and 108(T) were 35.1 and 36.4 mol%, respectively. Comparative 16S rRNA gene sequence analysis revealed that the isolates belonged to the genus Caldicellulosiruptor. However, DNA-DNA hybridization experiments indicated that the levels of relatedness between strains 2002(T) and 108(T) and those of recognized members of the genus Caldicellulosiruptor ranged between 32 and 54%. Based on both phenotypic and genomic differences, strains 2002(T) and 108(T) are considered to represent two novel species of the genus Caldicellulosiruptor. The names proposed for these organisms are Caldicellulosiruptor kronotskyensis sp. nov. (type strain 2002(T) =DSM 18902(T) =VKM B-2412(T)) and Caldicellulosiruptor hydrothermalis sp. nov. (type strain 108(T) = DSM 18901(T) =VKM B-2411(T)).