Cohnella phaseoli sp nov., isolated from root nodules of Phaseolus coccineus in Spain, and emended description of the genus Cohnella

A bacterial strain designated GSPC1(T) was isolated from root nodules of Phaseolus coccineus in Segovia (Spain). The 16S rRNA gene sequence of this strain showed 95.9 and 94.7 % sequence similarity, respectively, with those of the type strains of Cohnella hongkongensis and Cohnella thermotolerans. Strain GSPC1(T) presented phenotypic, chemotaxonomic and molecular differences with respect to Cohnella species which indicated that it belonged to a different species. The isolate was a Gram-positive, aerobic, sporulated rod, motile by means of peritrichous flagella. The strain was catalase-positive and showed weak oxidase activity. It grew in the presence of 2% NaCl. MK-7 was the predominant menaquinone, anteiso-C-15:0, iso-C-15:0, iso-C-16:0 and C-16:0 were the major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The G+C content was 60.3 mol%. The results of this study suggest that isolate GSPC1(T) should be classified within a novel Cohnella species, for which the name Cohnella phaseoli sp. nov. is proposed, with strain GSPC1(T) (=LMG 24086(T) DSM 19269(T)) as the type strain.