Chryseobacterium ureilyticum sp nov., Chryseobacterium gambrini sp nov., Chryseobacterium pallidum sp nov and Chryseobacterium molle sp nov., isolated from beer-bottling plants

Four Gram-negative, rod-shaped, non-spore-forming and non-motile bacterial strains were isolated from surfaces and biofilms associated with beer-bottling plants. Based on their 16S rRNA gene sequences these isolates were allocated to the genus Chryseobacterium. The sequence similarities of the isolates to the next most closely related type strains of this genus ranged from 96.4 to 98.3%. The presence of menaquinone MK-6 and predominant fatty acids 15: 0 iso, 17: 1 iso cis9, 15: 0 iso 2-OH and 17: 0 iso 3-OH supported the affiliation of these strains to the genus. The results of DNA-DNA hybridization, biochemical tests and chemotaxonomic properties allowed genotypic and phenotypic differentiation of the strains from the next most closely related Chryseobacterium species with validly published names. Therefore, the isolates represent four novel species for which the names Chryseobacterium ureilyticum (type strain F-Fue-04IIIaaaa(T) =DSM 18017(T) = CCUG 52546(T)), Chryseobacterium gambrini (type strain 5-1 St1a(T) = DSM 18014(T) = CCUG 52549(T)), Chryseobacterium pallidum (type strain 26-3St2b(T) = DSM 18015(T) = CCUG 52548(T)) and Chryseobacterium molle (type strain DW3(T) = DSM 18016(T) = CCUG 52547(T)) are proposed.