4.4 Article

Burkholderia sabiae sp nov., isolated from root nodules of Mimosa caesalpiniifolia

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MICROBIOLOGY SOC
DOI: 10.1099/ijs.0.65816-0

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  1. National Science Council, Taipei, Taiwan, Republic of China [96-2320-B-022-001-MY2, 96-2313-B-022-001]
  2. Natural Environment Research Council (NFRC), UK [NE/B505038/1, NE/B505046/1]
  3. Natural Environment Research Council [NE/B505038/1, NE/B505046/1] Funding Source: researchfish

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Two rhizobial strains, Br3407(T) and Br3405, were isolated from nitrogen-fixing nodules on the roots of Mimosa caesalpiniifolia, a legume tree native to Brazil. On the basis of 16S rRNA gene sequence similarities, both strains were shown previously to belong to the genus Burkholderia. A polyphasic approach, including DNA-DNA hybridizations, pulsed-field gel electrophoresis of whole-genome DNA profiles, whole-cell protein analyses, fatty acid methyl ester analysis and extensive biochemical characterization, was used to clarify the taxonomic position of these strains further; the strains are here classified within a novel species, for which the name Burkholderia sabiae sp. nov. is proposed. The type strain is strain Br3407(T) (=LMG 24235(T) =BCRC 17587(T)).

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