Journal
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Volume 58, Issue -, Pages 2305-2308Publisher
MICROBIOLOGY SOC
DOI: 10.1099/ijs.0.64947-0
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Funding
- Thailand Tropical Diseases Research programme [02-2-LEP-02-027]
- National Center for Genetic Engineering and Biotechnology
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A single Leptospira strain (designated Khorat-H2(T)) was isolated from the urine of an adult male patient with suspected leptospirosis from the province of Nakornrachasima, Thailand, The isolate showed typical Leptospira motility and morphology under dark-field microscopy. Cells were 10-13 mu m long and 0.2 mu m in diameter, with a wavelength of 0.5 mu m and an amplitude of approximately 0.3 mu m. Phenotypically, strain Khorat-H2(T) did not grow at 13 degrees C but grew at 30 and 37 degrees C and in the presence of 8-azaguanine. Serological identification using the microscopic agglutination test revealed that strain Khorat-H2(T) had no cross-reaction with any recognized Leptospira serogroups. Phylogenetic analysis of the 16S rRNA gene sequence placed the novel strain within the radiation of the genus Leptospira, with sequence similarities of 88.1-97.7% to recognized Leptospira species. DNA-DNA hybridization against the type strains of the three most closely related Leptospira species was used to confirm the results of the 16S rRNA sequence analysis. The G + C content of strain Khorat-H2(T) was 41.8 mol%. On the basis of phenotypic, serological and phylogenetic data, strain Khorat-H2(T) represents a novel species of the genus Leptospira, for which the name Leptospira wolffii sp. nov. is proposed. The type strain is Khorat-H2(T) (-WHO LT1686(T) =KIT Khorat-H2(T)).
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