Micromonospora chaiyaphumensis sp nov., isolated from Thai soils

Three actinomycete strains, MC5-1(T), MC7-1 and R1-1, were isolated from soil samples collected in Thailand. Their taxonomic positions were determined using a polyphasic approach. The chemotaxonomic characteristics of these strains coincided with those of the genus Micromonospora, i.e. meso-diaminopimelic acid and N-glycolyl muramic acid were present in the cell-wall peptidoglycan, the whole-cell sugars were of pattern D, the phospholipids were of type 11 and the cellular fatty acids were of type 3b. Phylogenetic analysis of the 16S rRNA gene sequences revealed a close relationship between strains MC5-1(T), MC7-1 and R1-1 (99.8% sequence similarity) and Micromonospora auratinigra JCM 12357(T) (99.3 %). The three novel strains were clearly distinguishable from M. auratinigra JCM 12357(T) from the low DNA-DNA relatedness (<= 43.4%). On the basis of the data presented, strain MC5-1(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora chaiyaphumensis is proposed. The type strain is MC5-1(T) (=KCTC 19332(T)=JCM 12873(T)= PCU 267(T) =TISTR 1564(T)).