Journal
INTERNATIONAL JOURNAL OF STD & AIDS
Volume 22, Issue 7, Pages 373-375Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1258/ijsa.2009.009526
Keywords
HIV-1; viral load; antiretroviral treatment; monitoring; resource-limited; realtime polymerase chain reaction
Categories
Funding
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD
- National Cancer Institute, National Institutes of Health [HHSN261200800001E]
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The need for viral load (VL) monitoring of HIV patients receiving antiretroviral therapy (ART) in resource-limited settings (RLS) has become apparent with studies showing the limitations of immunological monitoring. We compared the Abbott m2000 Real-Time (Abbott) HIV-1 assay with the Roche AMPLICOR Monitor v1.5 (Roche) HIV-1 assay over a range of VL concentrations. Three hundred and eleven plasma samples were tested, including 164 samples from patients on ART >= six months and 147 from ART-naive patients. The Roche assay detected >= 400 copies/mL in 158 (50.8%) samples. Of these, Abbott produced 145 (91.8%) detectable results >= 400 copies/mL; 13 (8.2%) samples produced discrepant results. Concordance between the assays for detecting HIV-1 RNA >= 400 copies/mL was 95.8% (298/311). The sensitivity, specificity, positive predictive value and negative predictive value of Abbott to detect HIV-1 RNA >= 400 copies/mL were 91.8%, 100%, 100% and 92.2%, respectively. For the 151 samples with HIV-1 RNA >= 400 copies/mL for both assays, a good linear correlation was found (r = 0.81, P < 0.0001; mean difference, 0.05). The limits of agreement were -0.97 and 1.07 log(10) copies/mL (mean +/- 2 SD). The Abbott assay performed well in our setting, offering an alternative methodology for HIV-1 VL for laboratories with realtime polymerase chain reaction (PCR) capacity.
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