CD4+Foxp3+ Tregs protect against innate immune cell-mediated fulminant hepatitis in mice

Foxp3(+) Tregs play important roles in maintaining homeostasis by suppressing excessive immune responses that result in serious tissue damage; yet, it is largely unknown about the impact of Tregs on innate immune cells in hepatitis models in vivo. In this study, we examined the effect of hepatic Tregs on innate immune-mediated liver injury by using the murine model of polyI:C and D-galactosamine (D-GalN)-induced hepatitis. Administration of polyI:C/D-GalN increased the number of CD4(+)Foxp3(+) Tregs in the liver. Depletion of Tregs leaded to higher levels of proinflammatory cytokine expression and severer liver injury, whereas adoptive transfer of Foxp3(+) Tregs attenuated liver injury in polyI:C/D-GalN-treated mice. In addition, depletion of Tregs leaded to a reduction in TGF-beta and IL-10 expression in polyI:C/D-GalN-treated mice. Both of these cytokines were important for suppression of polyI:C/D-GalN-induced liver injury. TGF-beta was derived from Tregs. IL-10 was derived from active Kupffer cells, and coincubation of Kupffer cells with Tregs increased IL-10 secretion. Furthermore, TGF-beta blockade abrogated Treg-mediated suppression of proinflammatory cytokine production by innate immune cell in vitro. Conclusion: CD4(+)Foxp3(+) Tregs modify innate immune responses in polyI:C/D-GalN-induced fulminant hepatitis via producing TGF-beta and enhancing IL-10 secretion by Kupffer cells. (C) 2014 Elsevier Ltd. All rights reserved.