Journal
MOLECULAR GENETICS AND GENOMICS
Volume 290, Issue 4, Pages 1393-1402Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00438-015-1003-0
Keywords
Heterozygosity; Population structure; Association mapping; Fruit quality; Bacterial blight; Pomegranate
Funding
- Indian Council of Agricultural Research (ICAR)
- Gus R. Douglass Institute of West Virginia State University
- National Agricultural Innovation Project, ICAR
- Office of Integrative Activities
- Office Of The Director [1003907] Funding Source: National Science Foundation
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This genetic diversity study aimed to estimate the population structure and explore the use of association mapping strategies to identify linked markers for bacterial resistance, growth and fruit quality in pomegranate collections from India. In total, 88 accessions including 37 cultivated types were investigated. A total of 112 alleles were amplified by use of 44 publicly available microsatellites for estimating molecular genetic diversity and population structure. Neighbor-joining analysis, model-based population structure and principal component analysis corroborated the genetic relationships among wild-type and cultivated pomegranate collections from India. Our study placed all 88 germplasm into four clusters. We identified a cultivated clade of pomegranates in close proximity to Daru types of wild-type pomegranates that grow naturally near the foothills of the Himalayas. Admixture analysis sorted various lineages of cultivated pomegranates to their respective ancestral forms. We identified four linked markers for fruit weight, titratable acidity and bacterial blight severity. PGCT001 was found associated with both fruit weight and bacterial blight, and the association with fruit weight during both seasons analyzed was significant after Bonferroni correction. This research demonstrates effectiveness of microsatellites to resolve population structure among the wild and cultivar collection of pomegranates and future use for association mapping studies.
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