Journal
MOLECULAR ENDOCRINOLOGY
Volume 29, Issue 11, Pages 1634-1645Publisher
ENDOCRINE SOC
DOI: 10.1210/me.2015-1153
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Funding
- National Institutes of Health [R01CA172437]
- Marie Curie Actions Grant FP7-PEOPLE-COFUND via the VINNOVA program Mobility for Growth [GROWTH 291795]
- University of Houston
- Texas Emerging Technology Fund [300-9-1958]
- McNair Foundation
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Estrogen receptor (ER)alpha-positive tumors are commonly treated with ER alpha antagonists or inhibitors of estrogen synthesis, but most tumors develop resistance, and we need to better understand the pathways that underlie the proliferative and tumorigenic role of this estrogen-activated transcription factor. We here present the first single-molecule sequencing of the estradiol-induced ER alpha transcriptome in the luminal A-type human breast cancer cell lines MCF7 and T47D. Sequencing libraries were prepared from the polyadenylated RNA fraction after 8 hours of estrogen or vehicle treatment. Single-molecule sequencing was carried out in biological and technical replicates and differentially expressed genes were defined and analyzed for enriched processes. Correlation analysis with clinical expression and survival were performed, and follow-up experiments carried out using time series, chromatin immunoprecipitation and quantitative real-time PCR. We uncovered that ER alpha in addition to regulating approximately 2000 protein-coding genes, also regulated up to 1000 long noncoding RNAs (lncRNAs). Most of these were up-regulated, and 178 lncRNAs were regulated in both cell lines. We demonstrate that Long Intergenic Nonprotein Coding RNA 1016 (LINC01016) and LINC00160 are direct transcriptional targets of ER alpha, correlate with ER alpha expression in clinical samples, and show prognostic significance in relation to breast cancer survival. We show that silencing of LINC00160 results in reduced proliferation, demonstrating that lncRNA expression have functional consequences. Our findings suggest that ER alpha regulation of lncRNAs is clinically relevant and that their functions and potential use as biomarkers for endocrine response are important to explore.
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