Journal
MOLECULAR ECOLOGY RESOURCES
Volume 16, Issue 1, Pages 176-182Publisher
WILEY
DOI: 10.1111/1755-0998.12428
Keywords
biodiversity; next-generation sequencing; PCR errors; sequence analysis; taxonomic annotation
Funding
- Agence Nationale de la Recherche (ANR) through the METABAR
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DNA metabarcoding offers new perspectives in biodiversity research. This recently developed approach to ecosystem study relies heavily on the use of next-generation sequencing (NGS) and thus calls upon the ability to deal with huge sequence data sets. The OBITOOLS package satisfies this requirement thanks to a set of programs specifically designed for analysing NGS data in a DNA metabarcoding context. Their capacity to filter and edit sequences while taking into account taxonomic annotation helps to set up tailor-made analysis pipelines for a broad range of DNA metabarcoding applications, including biodiversity surveys or diet analyses. The OBITOOLS package is distributed as an open source software available on the following website: http://metabarcoding.org/obitools. A Galaxy wrapper is available on the GenOuest core facility toolshed: http://toolshed.genouest.org.
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