Journal
MOLECULAR CELL
Volume 60, Issue 4, Pages 584-596Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2015.10.025
Keywords
-
Categories
Funding
- Ministry of Education, Science, Sports and Culture (MEXT), Japan
- Grants-in-Aid for Scientific Research [14J05296, 26560395, 22229009, 26293071, 25640098, 26830136, 15H04707, 26116708] Funding Source: KAKEN
Ask authors/readers for more resources
Bivalent H3K4me3 and H3K27me3 chromatin domains in embryonic stem cells keep active developmental regulatory genes expressed at very low levels and poised for activation. Here, we show an alternative and previously unknown bivalent modified histone signature in lineage-committed mesenchymal stem cells and preadipocytes that pairs H3K4me3 with H3K9me3 to maintain adipogenic master regulatory genes (Cebpa and Pparg) expressed at low levels yet poised for activation when differentiation is required. We show lineage-specific gene-body DNA methylation recruits H3K9 methyltransferase SETDB1, which methylates H3K9 immediately downstream of transcription start sites marked with H3K4me3 to establish the bivalent domain. At the Cebpa locus, this prevents transcription factor C/EBPb binding, histone acetylation, and further H3K4me3 deposition and is associated with pausing of RNA polymerase II, which limits Cebpa gene expression and adipogenesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available