Journal
MOLECULAR CELL
Volume 58, Issue 4, Pages 575-585Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2015.04.028
Keywords
-
Categories
Funding
- NIH [DK063720, U01CA168370, U01MH105028]
Ask authors/readers for more resources
The most widely used approach for defining gene function is to reduce or completely disrupt its normal expression. For over a decade, RNAi has ruled the lab, offering a magic bullet to disrupt gene expression in many organisms. However, new biotechnological tools-specifically CRISPR-based technologies-have become available and are squeezing out RNAi dominance in mammalian cell studies. These seemingly competing technologies leave research investigators with the question: Which technology should I use in my experiment? This review offers a practical resource to compare and contrast these technologies, guiding the investigator when and where to use this fantastic array of powerful tools.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available